Sericin from the cocoons of Silkworm, Antheraea Mylitta (L) and Bombyx Mori (L) for the Reduction in Hydrogen Peroxide Induced Oxidative Stress in Feline Fibroblasts

Authors

  • Vitthalrao Bhimasha Khyade  (A).Sericulture Unit, Malegaon Sheti Farm, Agricultural Development Trust Baramati, Shardanagar, (Malegaon Khurd) Post Box No - 35, Baramati, Pune, Maharashtra, India , (B). Head, Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Tal. Baramati Pune, Maharashtra, India , (C). Dr. APIS", Shrikrupa Residence, Teachers Society, Malegaon Colony (Baramati) Pune, Maharashtra, India
  • Shinya Yamanaka  Senior Investigator, Gladstone Institute of Cardiovascular Disease 1650 OWENS STREET, SAN FRANCISCO, CA 94158

Keywords:

Antioxidant Activity , Fibroblast Cell Line, Hydrogen Peroxide, Oxidative Stress, Moracin

Abstract

The silk fiber of cocoons of silkworms of both the families consist of two major proteins, fibroin and sericin. The fibrion forms central core and sericin forms envelop. Fibroin and sericin, of mulberry silkworm, Bombyx mori deserve appreciable physioc-chemical properties and therefore, they are now recognized as excellent biomaterials in the field of tissue engineering, biotechnology and therapy. Sericin contributes about 20-30 per cent of total cocoon weight. It is characterized by its high content of serine and 18 amino acids, including essential amino acids. There are different methods of isolation of sericin from silk thread. Solubility, molecular weight, and gelling properties of sericin depend on the method of isolation. It has wide applications in pharmaceuticals and cosmetics such as, wound healing, bioadhesive moisturizing, antiwrinkle and antiaging. The present attempt is concerned with assessment of potential regarding antioxidant activity of Sericin from silk from cocoons of non-mulberry silkworm, Antheraea mylitta And mulberry silkworm, Bombyx mori (L) through the use of hydrogen peroxide induced stress in skin fibroblast cell line culture ( AH927). Cells treated with sericin exhibited significant cell viability comparable to that of control group (P ?0.05). The fibroblasts pre-incubated with sericin of both the species at 100 ng/ml had significantly decreased (P ?0.01) catalase activity. The oxidative stress through hydrogen peroxide was found responsible for significantincrease (P ?0.01) in the release of enzyme (LDH) in comparison with untreated control. The sericin treatment definitely serving to restore the original membrane integrity of the cells. The aqueous form of silk sericin, from a natural source like silkworm cocoon, serve as ideal antioxidant source and may be used to treat the cancer cells.

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International Journal of Scientific Research in Chemistry

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2018-09-30

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Vitthalrao Bhimasha Khyade, Shinya Yamanaka, " Sericin from the cocoons of Silkworm, Antheraea Mylitta (L) and Bombyx Mori (L) for the Reduction in Hydrogen Peroxide Induced Oxidative Stress in Feline Fibroblasts, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 3, Issue 4, pp.01-16, September-October-2018.