Manuscript Number : IJSRCH18346

Changes in the Mid gut enzyme activity in the fifth instar larvae of silkworm, Bombyx mori (L) fed with mulberry leaves treated with aqueous solution of seed powder of Syzigium cumini (L) [(Race: Bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)].

Authors(3) :-Kajal Appasaheb Pondkule, Manisha Mahendra Nalwade, Vitthalrao B. Khyade

Four different concentrations (10.0 ppm; 20.0 ppm; 40.0 ppm & 50.0 ppm) of the aqueous solution of seed powder of Syzigium cumini (L) concentrations was used to treat the leaves of mulberry & fed to the fifth instar larvae of bivoltine, crossbreed silkworm, Bombyx mori (L) [(Race: Bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)] for first four days of fifth instar larvae. The larvae fed with untreated & water treated leaves were also maintained. The midgut enzyme (protease & amylase) bioassays were carried out on fifth day. The velocity of biochemical reaction catalyzed by mid gut protease and midgut amylase in larvae fed with untreated mulberry leaves was found measured 02.593 units and 5.547 units respectively. The midgut protease activity in larvae fed with mulberry leaves treated with various concentrations (10.0 ppm; 20.0 ppm; 40.0 ppm & 50.0 ppm) of the aqueous solution of seed powder of Syzigium cumini (L) was found measured 3.217; 4.339; 4.476 and 5.793 units respectively. There was 24 to 123 percent increase in the mid gut protease activity through Syzigium treatment. The midgut amyase activity in experimental group larvae in attempt was found measured 6.864; 10.148; 10.319 and 10.483 units respectively. There was 23 to 88 percent increase in the mid gut protease activity through Syzigium treatment. The contents of seed powder of Syzigium cumini (L) serve to improve the digestibility & exert the influence of efficient metabolism in the fifth instar larvae of silkworm, Bombyx mori (L). The Syzigium seed powder treatment may gear overall biochemical constituency of silkworm larvae, through the significant improvement in the velocity of mid gut enzyme catalyzed biochemical reactions.

Authors and Affiliations

Kajal Appasaheb Pondkule
Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India
Manisha Mahendra Nalwade
Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India
Vitthalrao B. Khyade
Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India

Bombyx mori (L); Midgut Protease; Midgut Amylase; Syzigium cumuni (L).

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Publication Details

Published in : Volume 3 | Issue 4 | September-October 2018
Date of Publication : 2018-09-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 43-52
Manuscript Number : IJSRCH18346
Date Written : 2018-09-10
Publisher : Technoscience Academy

ISSN : 2456-8457

Suggested Citation :

Kajal Appasaheb Pondkule, Manisha Mahendra Nalwade, Vitthalrao B. Khyade , "Changes in the Mid gut enzyme activity in the fifth instar larvae of silkworm, Bombyx mori (L) fed with mulberry leaves treated with aqueous solution of seed powder of Syzigium cumini (L) [(Race: Bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)].", International Journal of Scientific Research in Chemistry (IJSRCH), ISSN : 2456-8457, Volume 3, Issue 4, pp.43-52, September-October.2018
URL : https://ijsrch.com/IJSRCH18346

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