The reflection of feeding the mulberry leaves treated with water solution of seed powder of Syzigium cumini (L) into Profiles of Protein (Total) in the fifth instar larvae of silk worm, Bombyx mori (L) Race - bivoltine, crossbreed: [(CSR6 x CSR26)] x [CSR2 x CSR27)].

Authors

  • Manisha Mahendra Nalwade  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India
  • Kajal Appasaheb Pondkule  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India
  • Vitthalrao B. Khyade  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati, Pune, Maharashtra, India

Keywords:

Bombyx Mori (L), Total Proteins, Syzigium Cumuni (L).

Abstract

The seed of the fruit is used in various alternative healing systems like Ayurveda , Unani and Chinese medicine. Wine and vinegar are also made from the fruit. It has a high source in vitamin A and vitamin C. The present Attempt is concerned with the analysis of effect of Syzigium cumini (L) seed powder treatment on the levels of protein in the silkworm, Bombyx mori (L). Aqueous solutions of seed powder of Syzigium cumini (L) of different strengths (10 ppm; 20 ppm; 40 ppm & 50 ppm) was utilized to treat the leaves of mulberry. Treated mulberry leaves were fed to the fifth instar larvae of bivoltine, crossbreed race of silkworm, Bombyx mori(L) [(CSR6 x CSR26)] x [CSR2 x CSR27)]. The feeding the larvae with treated leaves was carried for first four days. The larvae fed with untreated & water treated leaves were also maintained. Total protein bioassay was carried out on fifth day through the use of silk glands, fat bodies and haemolymph. The total protein content of salivary glands of untreated group of larvae was found measured 22.817 units. Syzigium treatment was found resulted into increase in the salivary gland proteins (total) from 21 to 104 percent. Fat body total protein was increased from 34 to 90 percents through the Syzigium treatment. The haemolymph total proteins were found increased from 22 to 68 percent through the Syzigium treatment. Treating the leaves of mulberry with Syzizium powder may have had effect on the increase in the levels of amino acids. It may be followed by accelerated rate of synthesis of protein in the body of fifth instar larvae of silkworm, Bombyx mori (L).

References

  1. Applebaum, S.W. (1985). Biochemistry of digestion. In: Comprehensive insect physiology (Ed. Kerkut, G.A. and Gilbert (I), 4:297-307. Pergamon press New York.
  2. Bernfeld, P. (1955). Amylase, a and b activity of 10,11epoxyfranesoic acid methyl ester. Life Science. 4: 2323-2331. In: Methods of Enzymology, Vol.I (Ed. Clockwik & Kalpin). Academic Press, New York.
  3. Bowers, Brik, Y.; Harpaz Bowers, W.S.; Fales, V.M.; Thompson, M.J. and Uebel,B.(1966). Juvenile & gonadotropic,J. ; Ishaya and Bhondi, A.(1962), Studies on proteolytic activity of beetle, Tenebrio molitor (L). J. Insect Physiol. 8: 417-429.
  4. Chougale, A.C.(1992). Influence of magnetic energy on silkworm, Bombyx mori(L). Ph.D. Thesis, Shivaji University, Kolhapur.
  5. Desai, V.A.; Pawar, V.V. &Sawant, R.T.(2011). Influence of herbal drug: kho-go on the fifth instar larvae of silkworm, Bombyx mori (L).Dissertation in the partial fulfillment of M.Sc. (Microbiology), Shardabai Pawar Mahila College, Shardanagar (Baramati), (Pune University, Pune).
  6. Gaikwad, A.R.(1998). Biology of some dung beetles of South Western Maharashtra. Ph.D. thesis, Shivaji University, Kolhapur.
  7. Ghantaloo, U.S. (2007). Influence of digoxin on silkworm, Bombyx mori (L). M.Phil. Thesis, Algappa University, Karaikudi (Tamil Nadu),India.
  8. Horie, Y.; Tanaka,M. and Ito,T. (1963). Proteolytic enzyme of digestive juice of mid gut in silkworm, Bombyx mori (L). J. Setricult. Sci. Japan, 32: 8-15.
  9. Horie, Y.(1961). Physiological studies on the alimentary canal of silkworm, Bombyxmori (L).III. Absorption& utilization of carbohydrates. Bull. Sericult. Exp. Sta. Japan, 16: 287-309.
  10. Ishaya,I. and Swirski, E. (1976). Trehalase, invertase& amylase activities in the larvae of Egyptian cotton worm, Spodoptera littoralis (L). J. Insect Physiol. 17: 945-953.
  11. Ito, H. (1960). Effect of sugars on feeding the larvae of silkworm, Bombyx mori (L) J. Insect. Physiol. Vol.5: 95-107.
  12. Ito, T. (1961). Nutrition of silkworm, Bombyx mori (L). Proc. Jpn. Acad. Sci. 43:57-61.
  13. Ito, T; Kwashima, K.; Nakhara,M.; Nakanshi, K. & Terahara, A. (1964). Metabolism in the mid gut of silkworm, Bombyx mori (L). Insect Physiol. Vol.10: 225-228.
  14. Ishaaya, I.; Moore, I and Joseph, B.(1971). Protease & amylase activity in the larvae of Egyptian cotton worm, Spodoptera littoralis (L). J. Insect physiol. 17: 945-953.
  15. Jagtap, S.G. (2007). Effect of plant juvenoids on consumption &utlizationof mulberry leaves by silkworm, Bombyx mori (L). M.Phil. Thesis, Algappa University, Karaikudi, Tamil Nadu, (India).
  16. Khyade, V.B.(2004). Influence of juvenoids on silkworm, Bombyx mori (L). Ph.D. thesis, Shivaji University, Kolhapur.
  17. Krishnaswami, S.; Narasimhna, M.N.; Suryanarayana, S.K. and Kumararaj, S.(1978). Sericulture Manual-II Silkworm Rearing: FAO. United Nations Rome.
  18. Lowery, O.H.; Rosenbrough, N.J.; Far,A.L. and Randall, R.J.(1951). Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275.
  19. Murali, K.(1992).Effect of leaf carbohydrate reserves on the growth& excretory pattern of silkworm, Bombyx mori (L). M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
  20. Murugan, K.and George, A. (Sr.)(1992). Feedings & nutritional influence on growth & reproduction of Daphnia near (L). Insect Physiol. 38: 961-969.
  21. Nayar, J.K.&Frankel,G.(1962). Journal of Insect Physiology. Volume-8, page-505.
  22. Norman, T.J.& Baily (1955). Statistical methods in Biology.
  23. Slansky,F. & Scriber, J.M. (1985). Food consumption & Utilization. In: comprehensive Insect physiology, Biochemistry & pharmacology. (Eds. Kerkut, G.A. and Gilbert, L.I.) 4, Pergamon Press, Oxford, Page: 639.
  24. Subramanyam Reddy, C.(1992).Studies on distribution of digestive enzymes in the digestive tract of silkworm, Bombyx mori(L). M.Phil. Dissertation, Sri. Venkateshwara University, Tirupati (India).
  25. VitthalraoKhyade and Jeevan P. Sarawade (2009).protein profiles in the fifth instar larvaeof silkworm, Bombyx mori (L)(PM xCSR2) fed with digoxin treated mulberry leaves. The Bioscan(1): 41-44.
  26. Vitthalrao B. Khyade & Jyoti Kulkarni (2011). Effect of digoxin treated mulberry leaves on protein profiles in fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR2). Res. J. Chem. Sci. Vol.1 (1): 2-7 (www.isca.in)
  27. Yamafuji, I. and Yonezawa (1935). Lipases in silkworm, Bombyx mori (L).Insect. Biochem. 1: 102-112.
  28. Mankiewicz, Richard (2004). The Story of Mathematics (Paperback ed.). Princeton, NJ: Princeton University Press. p. 158. ISBN 9780691120461.
  29. O'Connor, John J.; Robertson, Edmund F., "William Sealy Gosset", MacTutor History of Mathematics archive, University of St Andrews.
  30. Fisher Box, Joan (1987). "Guinness, Gosset, Fisher, and Small Samples". Statistical Science. 2 (1): 45–52. doi:10.1214/ss/1177013437. JSTOR 2245613.
  31. https://en.wikipedia.org/wiki/Student%27s_t-test

Downloads

Published

2018-09-30

Issue

Section

Research Articles

How to Cite

[1]
Manisha Mahendra Nalwade, Kajal Appasaheb Pondkule, Vitthalrao B. Khyade, " The reflection of feeding the mulberry leaves treated with water solution of seed powder of Syzigium cumini (L) into Profiles of Protein (Total) in the fifth instar larvae of silk worm, Bombyx mori (L) Race - bivoltine, crossbreed: [(CSR6 x CSR26)] x [CSR2 x CSR27)]., International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 3, Issue 4, pp.68-76, September-October-2018.