Utilization of the Aqueous Mulberry (Morus alba. L.) Leaf Decoction (AMLD) For Treating the DMBA Induced Hepatotoxicity and Free-Radical Damage in Norwegian Rat, Rattus norvegicus (L).

Authors

  • Vitthalrao B. Khyade  Head, Department of Zoology Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Tal. Baramati, Dist. Pune, Maharashtra, India
  • James P. Allison  Department of Immunology, MD Anderson Anderson Cancer Center, UTHealth Graduate School of Biomedical Sciences (The University of Texas), PO Box 20334, Houston TX 77225-0334

Keywords:

DMBA, Oxidative Stress, Antioxidants, Histopathology, Hepatotoxicity, AMLD

Abstract

The aqueous mulberry (Morus alba L.) leaf decoction (AMLD) is well documented for antioxidant activity. The present attempt deals with histological and biochemical evaluation of aqueous mulberry (Morus alba L.) leaf decoction (AMLD) for protective influence in the 7,12-Dimethylbenz[a]anthracene (DMBA) induced liver damage. The 7,12-Dimethylbenz[a]anthracene (DMBA) is acting as immuno suppressor and it serves as a tumor initiator. The promotion of cancer or tumor is possible through the treatments of 12-O-tetradecanoylphorbol-13-acetate (TPA) in some models of two-stage carcinogenesis. This TPA allows greatly accelerated rate of growth of tumor. Forty Norwegian Rats ( Rattus norvegicus L) were divided into four groups, each group with ten individuals. The groups incude: 1) Untreated control group – received oral corn oil; 2) DMBA treated group –received DMBA was orally 335 mg/kg in the corn oil solution; 3) Aqueous Mulberry Leaf Decoction (AMLD) Treated group – received 100 mg AMLD/kg/day was orally, at every 24 hours for 7 days and 4) The group treated with DMBA followed by AMLD (DMBA + AMLD). All animals rats sacrificed at the end of experiment. Bioassays of Superoxide dismutases (SOD), glutathione peroxidase (GPX), nitric oxide (NO); myeloperoxidase (MPO); aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were carried in serum and liver tissue. The histopathological study on liver tissue was evaluated with Hematoxilin & Eosin stains. Biochemical studies were found that, ALT, AST, NO, MPO in serum and NO, MPO in liver tissue significantly higher in DMBA group, compared to control group (P < 0.001). In Group AMLD + DMBA, serum AST, ALT, NO, MPO levels were significantly lower (P < 0.01), and both serum and tissue SOD activities were found significantly higher, compared to DMBA group (P < 0.001). Severe dilation; moderate disruption of the radial alignment of hepatocyte cytoplasm; inflammation around central vein and portal region were observed as DMBA induced histopathological changes in liver tissue. In rats receiving both DMBA and AMLD, the DMBA induced changes accounted for less sinusoidal dilatation, vacuolization in the hepatocyte cytoplasm and the inflammation around central vein and portal region (P < 0.05). The DMBA was found to induce liver damage by oxidative stress mechanisms. Aqueous Mulberry Leaf Decoction (AMLD) was reported to reduce the oxidative stress by inducing antioxidant mechanisms, thereby showing protective effect against DMBA induced liver damage

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Vitthalrao B. Khyade, James P. Allison, " Utilization of the Aqueous Mulberry (Morus alba. L.) Leaf Decoction (AMLD) For Treating the DMBA Induced Hepatotoxicity and Free-Radical Damage in Norwegian Rat, Rattus norvegicus (L)., International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 3, Issue 5, pp.106-123, November-December-2018.