A Study of Antioxidant Activities and GC-MS Analysis of Sodium nitroprusside (SNP) on Zea mays Leaves

Authors

  • S. Muthulakshmi  Department of Botany with specialization in Plant Biotechnology,The Standard Fireworks Rajaratnam College for Women,Sivakasi, Tamil Nadu, India
  • K. Lingakumar  Centre for Research and Postgraduate Studies in Botany,Ayya Nadar Janaki Ammal College (Autonomous ), Sivakasi, Tamil Nadu, India.

Keywords:

Antioxidant, Bioactive compounds, GC-MS, Sodium nitroprusside (SNP) and Zea mays

Abstract

The present study was conducted to investigate the foliar applied Sodium nitroprusside (SNP) on antioxidant and bioactive compounds in Zea mays. The plants where treated with different concentration of SNP (5µM and 1mM) on 20 days old seedling Zea mays. The treatment was carried out for 2 days. In general, the 5µM and 1mM increase in the production of proline content, superoxide dismutase (SOD), catalase (CAT), peroxidase (PD) and Malondialdehyde content when compare to the control. Where as in case of bioactive compounds production the maximum significant of bioactive compounds are present in 5µM of sodium nitroprusside. The major constituents were Octasiloxane 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15–hexadecamethyl (RT: 15.873); 2-Ethylacridine (RT: 16.582); Silicic acid, diethyl bis (trimethylsilyl)ester (RT: 17.395) along with other minor constituents were also present. Hence it is concluded that regular application of SNP to Zea mays resulted in higher biomass production and phytochemical content.

References

  1. Ayoub M, de Camargo AC, Shahidi, F. Antioxidants and bioactivities of free, esterified and insoluble-bound phenolics from berry seed meals. Food Chem 2016;197:221-32.
  2. Blomhoff R, Carlsen MH, Andersen LF, Jacobs DR. Health benefits of nuts: potential role of antioxidants. Br J Nutr 2006;96(S2):S52-60.
  3. Bowler, P.W., Jordan, P.W., Flint, W.G. and Caldwell, M.M. (1988). Competition, morphology and canopy structure in wheat (Triticum aestivum L.) and wild oat (Avena fatua L.) exposed to enhanced ultraviolet-B radiation. Funct. Ecol. 2: 391-330.
  4. Brewer MS. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf 2011;10:221-47.
  5. Fiedor J, Burda K. Potential role of carotenoids as antioxidants in human health and disease. Nutrients 2014;6:466-88.
  6. Finley JW, Kong AN, Hintze KJ, Jeffery EH, Ji LL, Lei XG. Antioxidants in foods: state of the science important to the food industry. J Agric Food Chem 2011;59:6837-46.
  7. Flora SJS. Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid Med Cell Longev 2009;2:191-206.
  8. Gungor N, Ozyurek M, Guclu K, Cekik SD, Apak R. Comparative evaluation of antioxidant capacities of thiol-based antioxidants measured by different in vitro methods. Talanta 2011;83:1650-8.
  9. Heath, R.L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics. 125: 189-198.
  10. Manai, J., Gouia, H., Corpas, F.J. (2014). Redox and nitric oxide homeostasis are affected in tomato (Solanum lycopersicum) roots under salinity-induced oxidative stress. J. Plant Physiol. 171: 1028-1035.
  11. Masaki H. Role of antioxidants in the skin: antiaging effects. J Dermatol Sci 2010;58:85-90.
  12. Mckenzie, L.O., Bjorn, L.O. and Caldwell, M.M. (1998). Changes in biologically active ultraviolet radiation reaching the earth's surface. Journal of Photochem and Photobiology B: Biology, 46: 5-19.
  13. Mooradian AD. Antioxidant properties of steroids. J Steroid Biochem Mol Biol 1993;45:509-11.
  14. Parviz , A., Satyawati, S.(2008). Salt stress and photobiochemical responses of plants- a review. Plant. 3: 1737-1747.
  15. Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000;63:1035-42.
  16. Torre GLTD, Arollado EC, Atienza AA, Manalo RAM. Evaluation of antioxidant capacity and identification of bioactive compounds of crude methanol extracts of Caesalpinia pulcherrima (L.) Swartz. Indian J Pharm Sci 2017;79:113-23.

Downloads

Published

2018-11-30

Issue

Section

Research Articles

How to Cite

[1]
S. Muthulakshmi, K. Lingakumar, " A Study of Antioxidant Activities and GC-MS Analysis of Sodium nitroprusside (SNP) on Zea mays Leaves, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 3, Issue 5, pp.67-72, November-December-2018.