Eco-friendly Synthesis, Characterization, and Antimicrobial Study of Mono Metallic Silver Nanoparticles from Khaya senegalensis Aqueous Stem Bark Extract

Authors

  • Mela Yoro  Department of Chemical Sciences, Federal University of Kashere, Gombe, Nigeria
  • Rebeccah Bethuel  Department of Microbiology, Federal University of Kashere, Gombe, Nigeria
  • Mary Asugu Mbahi  Chemistry Department, College of Science and Technology, Covenant University, Ogun, Nigeria
  • Robinson Ayibanuah Favour  

Keywords:

Eco-friendly Synthesis, Characterization, Antimicrobial, Monometallic, Nanoparticles, Khaya senegalensis

Abstract

Healthy Khaya senegalensis stem bark were collected from the Billiri area and were carefully washed under running water, rinsed multiple times with distilled water, and then allowed to dry in the shade to eliminate any remaining moisture. Using a mortar and pestle, the dried materials were ground into a fine powder which was used in making the extract. 10ml of Khaya senegalensis stem bark extract was added to 100ml of 0.01 M aqueous AgNO3 solution. On the hot plate, the mixture was heated to 60°C while being stirred continuously and after some time, AgNPs were formed. The UV analysis showed a maximum absorbance of 2.926 AU at a matching wavelength (? max) of 400 nm. An OH bond stretching may be the cause of a peak at 1845.08 cm-1. The shoulder peak of aldehydes belongs to the C=O group and is found at 1651.32 cm-1. The peak at 1314.99 cm-1 displays the fingerprint region of functional groups such as carboxylic and alcohol groups. The absorption peak at 1089.75 cm-1 could be the result of the CH2-CH3 bend. The FTIR analysis reveals that the aldehyde (C=O), hydroxyl (OH), and alkane (C-H) are the main agents in the conversion of Ag+ to g0 nanoparticles. The XRD result revealed that, the nanoparticles under study has face-centered cubic (fcc) and spinel-like structures, with biosynthesized AgNPs having an average particle size of 55.4 nm. Silver nanoparticles were tested for their antibacterial properties against two fungi, Candida albicans and A. niger, as well as gram-positive bacteria, K. pneumonia, and B. subtilis. several concentrations of silver nanoparticles were examined (100, 200, 300, 400, and 500 ?g/L) against each pathogen.The inhibitory zone grows as silver nanoparticle concentration increases. The zones of inhibition for B. subtilis, K. pneumonia, A. niger, and C. albicans were 14.5 mm, 25.3 mm, 23.5 mm, and 19.5 mm, respectively, at a higher concentration of 500?g/L. Compared to all other pathogens under research, K. pneumonia showed a higher zone of inhibition for each concentration examined. The study's findings therefore showed that silver nanoparticles made from Khaya senegalensis stem bark extract had strong antibacterial activity against the pathogenic microorganisms investigated.

References

  1. Samuel, P., Akintunde, S., Iliya, D.B., Sani, I.A., Muawiyya, M.M. (2022). Biological synthesis and characterization of copper oxide nanoparticles using aqueous Psidium guajava leave extract and study of antibacterial activity of the copper oxide nanoparticles on Escherichia coli and Staphylococcus aureus. Comprehensive Research and Reviews in Chemistry and Pharmacy, 2022, 01(01), 033–039 
  2. Mela Y., Bertha A.D., Rebeccah B., Japhet J., Joyous W.K. J., Suleiman T.A(2023). Plant Based Green Synthesis, Characterization and Antimicrobial Investigation of Silver Nanoparticles from Khaya senegalensis Aqueous Leaf Extract. International Journal of Scientific Research in Chemistry(IJSRCH), 8(5): 2456-8457
  3. Mela Y., Amos G., and Joyous W.K.J (2023). Characterization and Pathogenic Study of Plant Mediated Silver Nanoparticles Utilizing Psidium guajava Stem Bark Extract. International Journal of Scientific Research in Chemistry(IJSRCH), 8(1): 2456-8457
  4. Gnanajobitha, G., Annadurai, G., & Kannan, C. (2012). Green synthesis of silver nanoparticle using Elettaria cardamomom and assessment of its antimicrobial activity. Int. J. Pharma Sci. Res.(IJPSR), (3)3: 323–330
  5. Siddiqi, K. S., Husen, A., & Rao, R. A. K. (2018). A review on biosynthesis of silver nanoparticles and their biocidal properties. J. Nanobiotechnology, 16(1):018-033
  6. Mela, Y., John, D.S., Japhet, J., Joyous, W.K.J., Patrick, D.B., Dangiwa, G.A., & Nazifa, U. (2022). Biosynthesis, optimization of process parameters and antimicrobial activity of silver nanoparticles from Moringa oleifera leaf extract. Comprehensive Research and Reviews in Chemistry and Pharmacy, 2022, 01(01), 001–011
  7. Badnore, A. U., Sorde, K. I., Datir, K. A., Ananthanarayan, L., Pratap, A. P., & Pandit, A. B. (2019). Preparation of antibacterial peel-off facial mask formulation incorporating biosynthesized silver nanoparticles. Appl. Nanosci.9 (2): 279–287
  8. Ramasamy, M., & Lee, J. (2016). Recent nanotechnology approaches for prevention and treatment of biofilm-associated infections on medical devices. Biomed Res. Int., vol. 2016, 2016, doi: 10.1155/2016/1851242.
  9. Pestovsky, Y. S., & Martinez-Antonio, A. (2017) The use of nanoparticles and Nano formulations in agriculture J. Nanosci. Nanotechnology, 17(12): 8699–8730
  10. Mochi, F., et al., “Interaction of Colloidal Silver Nanoparticles with Ni2+: Sensing Application,” Proceedings, vol. 1, no. 10, p. 427, 2017, doi: 10.3390/proceedings1040427.
  11. Shen, Z., Han, G., Liu, C., Wang, X., & Sun, R (2016). Green synthesis of silver nanoparticles with bagasse for colorimetric detection of cysteine in serum samples. J. Alloys Compd., vol. 686, no. October, pp. 82–89, 2016, doi: 10.1016/j.jallcom.2016.05.348.
  12. Ghodake, G., Shinde, S., G., Saratale, G.D., Kadam, A., Saratale, R.G., & Kim, D.Y. (2020). Water Purification Filter Prepared by Layer-by-layer Assembly of Paper Filter and Polypropylene-polyethylene Woven Fabrics Decorated with Silver Nanoparticles. Fibers Polymer. 21(4):751–761
  13. Patel, A., Sharma, D., Kharkar, P., & Mehta, D. (2019). Application of Activated Carbon in Waste Water Treatment. International Journal of Engineering Applied Sciences and Technology, 3(12): 63–66
  14. K. N and S. M, “Efficient Removal of Toxic Textile Dyes using Silver Nanocomposites,” J. Nanosci. Curr. Res., vol. 02, no. 03, pp. 2–6, 2017, doi: 10.4172/2572-0813.1000113.
  15. Doc Brown’s.chemistry Revision Notebook dopamine. Journal of sensors and Actuators B. 2011; 93-102.
  16. Daniel, M.C., Astruc D. (2004). Gold nanoparticle; Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. J. Chem. Rev. 2004; 104: 293-396.
  17. Perez, J., Bax, L., Escolano, C. (2005). Roadmap report on Nanoparticles; Willemsy and Van Den Wilddenberg: Barcelona, spain. 2005.
  18. Mohanpuria, N.K., Rama, S.K., Yadav. (2008). Biosynthesis of nanoparticles: technology concepts for feature Application. J.NPS. 6(10): 507-517.
  19. Jhaa, A., Prasad K. (2009). A green low-cost biosynthesis of Sb2O3 nanoparticles43(3): 303-306
  20. Mela, Y., Japhet, J., Ayuba, I., Joyous, W. K. J., Patrick, D. B. (2022). Green Synthesis, Characterization and Antibacterial Activity of Cobalt Nanoparticles from Parkia biglobosa Aqueous Stem Extract. Scholars International Journal of Chemistry and Material Sciences, 5(5): 79-85
  21. Mela, Y., John, D. S., Japhet, J., Patrick, D. B., Joyous, W. K. J. (2022). Physicochemical Parameters and Antibacterial Activity of Biosynthesized Silver Nanoparticles from Carica papaya Leaf Extract. Scholars International Journal of Chemistry and Material Sciences, 5(6): 105-110
  22. Adelere I.A., Lateef, A. (2016). A novel approach to the green synthesis of metallic nanoparticles: The use of agro-wastes, enzymes, and pigments. Nanotechnology. Rev., 5(6): 567–587
  23. Jabna, K.K., Meera,V. (2017). Nano silver as Antimicrobial Agent in Treatment of Water / Waste Water. International Conference on Innovative Research in Science, Technology and Management, 3(1): 399–406.
  24. Mela, Y., Ayuba, I., Japhet, J. (2022). Evaluation of Antibacterial Efficacy of Green Synthesized Silver Nanoparticles from Moringa Oliefera Aqueous Root Extract. Int. j. adv. multidisc. res. stud. 2(3):61-66 
  25. Mohammed, R.S., Mujeeb, K., Mufsir, K., Abdulrahman, A., Warthan, Hamad Z. Alkhathlan, Mohammed Rafiq H. Siddiqui, Jilani P. Shaik, Anis Ahamed, Adeem Mahmood, Merajuddin Khan, and Syed Farooq A. (2018). Plant-Extract-Assisted Green Synthesis of Silver Nanoparticles Using Origanum vulgare L. Extract and Their Microbicidal Activities. Sustainability 2018, 10, 913
  26. Mela, Y., Ayuba, I., Japhet, J. (2022). Evaluation of Antibacterial Efficacy of Green Synthesized Silver Nanoparticles from Moringa Oliefera Aqueous Root Extract. Int. j. adv. multidisc. res. stud. 2022; 2(3):61-66
  27. Samaira, Y., Shazia, N., Haq Nawaz, B., Dure, N.I., Shan, I., Junaid, M., Rahat, M., Numrah, N., Jan, N., Arif, N., Munawar, I., and Hina, R. (2020). Green synthesis, characterization and photocatalytic applications of silver nanoparticles using Diospyros lotus. Green Process Synth, (9): 87–96
  28. Igwe, O. U., & Ekebo, E. S. (2018). Biofabrication of cobalt Nanoparticles using leaf extract of Chromolaena odorata and their potential antibacterial application, Research Journal of Chemical Sciences, 8(1), 11-17.
  29. Mela, Y., John, D.S., Japhet, J., Joyous, W.K.J., Patrick, D.B., Dangiwa, G.A., & Nazifa, U. (2022). Biosynthesis, optimization of process parameters and antimicrobial activity of silver nanoparticles from Moringa oleifera leaf extract. Comprehensive Research and Reviews in Chemistry and Pharmacy, 2022, 01(01), 001–011

International Journal of Scientific Research in Chemistry

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Published

2024-03-30

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Volume 9, Issue 2, March-April-2024

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Research Articles

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How to Cite

[1]
Mela Yoro, Rebeccah Bethuel, Mary Asugu Mbahi, Robinson Ayibanuah Favour, " Eco-friendly Synthesis, Characterization, and Antimicrobial Study of Mono Metallic Silver Nanoparticles from Khaya senegalensis Aqueous Stem Bark Extract, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 9, Issue 2, pp.01-09, March-April-2024.