The Activity of Midgut Enzymes in the Fifth Instar Larvae of Silk Worm, Bombyx Mori (L) Fed with mulberry leaves treated with Water Solution of Eurhodin

Authors

  • Samiksha Sunil Pisal  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Tal. Baramati Dist. Pune, Maharashtra, India
  • Amruta Chandrakant Nimbalkar  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Tal. Baramati Dist. Pune, Maharashtra, India
  • Vitthalrao B. Khyade  Science Association, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Tal. Baramati Dist. Pune, Maharashtra, India

Keywords:

Bombyx mori (L), Midgut Protease, Midgut Amylase, Eurhodin.

Abstract

Eurhodin is well known as vital dye and used for addition to some growth media for bacterial and cell cultures. The present Attempt is concerned with the analysis of effect of Eurhodin treatment on the magnitude of velocity of midgut enzymes (protease and amylase) in the homogenate of mid gut tissue of fifth instar larvae of silkworm, Bombyx mori (L). Aqueous solutions of Eurhodin of different strengths (25 ppm; 50 ppm; 75 ppm & 100 ppm) was utilized to treat the leaves of mulberry. 400 ml of aqueous solution of Eurhodin powder was used to treat 100 grams of fresh mulberry leaves for feeding the group of hundred larvae for each time. Treated mulberry leaves were fed to the fifth instar larvae. The feeding the larvae with treated leaves was carried for first four days. Four feedings was the schedule for each day. For each feeding hundred grams of leaves were used. The larvae fed with untreated & water treated leaves were also maintained. The bioassay of mid gut enzymes was carried out on fifth day through the use of mid gut homogenate. The velocity of biochemical reaction catalyzed by mid gut protease and midgut amylase in larvae fed with untreated mulberry leaves was found measured 03.057 (±0.365) units and 6.539 (±0.918) units respectively. The midgut protease activity in larvae fed with mulberry leaves treated with various concentrations (25.0 ppm; 50.0 ppm; 75.0 ppm & 100.0 ppm) of the aqueous solution of Eurhodin powder was found measured 3.793 (±0.696); 5.115 (±1.109); 5.276 (±1.789) and 6.817 (±2.013) units respectively. There was 24.075 to 122.99 percent increase in the mid gut protease activity through Eurhodin treatment. The midgut amyase activity in experimental group larvae in attempt was found measured 6.869 (±1.217); 07.549 (±2.329); 07.719 (±3.313) and 08.539 (±3.789) units respectively. There was 05.046 to 30.585 percent amylase in the mid gut protease activity through Eurhodin treatment. The Eurhodin contents serve to improve the digestibility & exert the influence of efficient metabolism in the fifth instar larvae of silkworm, Bombyx mori (L). The Eurhodin powder treatment may gear overall biochemical constituency of silkworm larvae, through the significant improvement in the velocity of mid gut enzyme catalyzed biochemical reactions.

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International Journal of Scientific Research in Chemistry

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2019-03-30

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Volume 4, Issue 2, March-April-2019

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[1]
Samiksha Sunil Pisal, Amruta Chandrakant Nimbalkar, Vitthalrao B. Khyade, " The Activity of Midgut Enzymes in the Fifth Instar Larvae of Silk Worm, Bombyx Mori (L) Fed with mulberry leaves treated with Water Solution of Eurhodin, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 4, Issue 2, pp.22-34, March-April-2019.