HPTLC finger printing studies and evaluation of Pharmacopoeial Standards for the medicinal plant Adiantum capillus-veneris L.

Authors

  • Pawan Kumar Sagar  Drug Standardization Research Institute, Ghaziabad, CCRUM., Ministry of AYUSH. Govt. of India
  • M Alam  Central Council for Research in Unani Medicine, New Delhi, Ministry of AYUSH. Govt. of India
  • S Sajwan  Drug Standardization Research Institute, Ghaziabad, CCRUM., Ministry of AYUSH. Govt. of India
  • A S Khan  Drug Standardization Research Institute, Ghaziabad, CCRUM., Ministry of AYUSH. Govt. of India
  • R P Meena  Central Council for Research in Unani Medicine, New Delhi, Ministry of AYUSH. Govt. of India

Keywords:

Adiantum capillus-veneris L., pharmacological characters, physico-chemical analysis, TLC/HPTLC research studies, Quality Assurance, Quality Control parameters.

Abstract

Adiantum capillus-veneris L. is commonly known as herbaceous plant belong to the family Adiantaceae. It is an important medicinal plant native to the United States America, Eurasia, the Levant in Western Asia, Australasia and Asian region, North east India region and west Bengal etc. The aerial parts of the plant were collected freshly and subjected to macroscopic, microscopic, physico-chemical and quality assurance, quality control parameters studies to evaluate and fix the drug validation, authenticate quality standards development. Active phytochemical constituents present in the plant triterpenoids, aoleananes, phenyl propaniods, carbohydrates, carotenoids,alicyclics and flavonoids like rutin, quercetin, quercetin-3-O-glucoside, querciturone, soquercitrin, nicotiflorin, naringin, astragalin, populnin, procyanidin, prodelphinidin, and kaempferol-3-sulfate. The physico-chemical data showed foreign matter %, w/w.-0.48, moisture content %,w/w.-2.856, total ash % w/w.-6.806,acid insoluble ash %,w/w.-3.630 and alcohol, water and hexane soluble extractive values, %,w/v.- 12.23,13.33 and 2.823, pH (1% &10% aq. solution)-6.92 & 6.18 respectively. TLC/ HPTLC studies of chloroform and alcohol extracts showed various spots / peaks at 254nm, 366nm and derivatized plates (Vanillin-sulphuric acid reagent), Quality assurance and Quality control parameters such as microbial content, heavy metals (As, Cd, Pb and Hg ppm.), Aflatoxins (B1.B2,G1 and G2 ppb.) were detected to be within the permissible limits. The study will be useful for the identification and authentication of the plant in dry form as well as in fresh form. The evaluated phytochemical and HPTLC. research data’s will serve as referential supports of pharmacopoeial standard research development of the plant in the near future for any analytical and biological research studies.

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International Journal of Scientific Research in Chemistry

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Published

2022-05-30

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Volume 7, Issue 3, May-June-2022

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Research Articles

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[1]
Pawan Kumar Sagar, M Alam, S Sajwan, A S Khan, R P Meena, " HPTLC finger printing studies and evaluation of Pharmacopoeial Standards for the medicinal plant Adiantum capillus-veneris L., International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 7, Issue 3, pp.01-15, May-June-2022.