Anthelmintic activity of Zingiber roseum rhizomes against Pheretima posthuma: In vitro and in silico approach

Authors

  • Muhammed Amanat  Department of Phrmacy, Eastern University, Bangladesh
  • Muhammad Tawhid  Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University
  • A.F.M Shahid-Ud-Daula  Department of Phrmacy, Noakhali Science and Technology University

Keywords:

Zingiber roseum, Tannins, Anthelmintic, Molecular docking, QSAR prediction

Abstract

Background : The goal of this study was to investigate the anthelmintic activity of a methanol extract of Zingiber roseum rhizome (ZRR) in an experimental model, followed by an in silico molecular docking study and an ADMET analysis. Methods : A worm called Pheretima posthuma was used to test the anthelmintic activity.Then, molecular docking study was performed of identified compounds of Z.roseum rhizome from HPLC namelycatechin hydrate, epicatechin, trans-ferulic acid, rosmarinic acid,quercetin, myricetin, trans-cinnamic acid having maximum activity against ?-TUBULIN enzyme by using CB-Dock protein-ligand docking server. Additionally, DruLiTo software was used to investigate drug-like predictions, and ADME/T profiles were examined using Swiss ADME Analysis and Molinspiration Cheminformatics software. Results : ZRR contained alkaloids, flavonoids, tannins, and saponins, according to preliminary phytochemical analysis. On an adult worm Pheretima posthuma, ZRR exhibited dose-dependent and statistically significant anthelmintic activityThe best concentration of ZRR for anthelmintic activity on Pheretima posthuma was compared to the reference standard Albendazole (2.5 & 5.0 mg/ml).Our molecular docking study, on the other hand, reveals that the phenolic compounds (except trans-cinnamic acid) have strong binding affinities (ranging from -6.4 to -8.5 KCal/mol) to ?-tubulin.These phyto-compounds from natural sources could be used to develop a reliable medication or to aid in lead identification using modern techniques. Conclusion : The current study's findings confirmed Zingiber roseum rhizome extract's potential anthelmintic activity, and all compounds (except trans-cinnamic acid) were found to be effective in computer-aided drug design models.

References

  1. Chan, M., et al., The evaluation of potential global morbidity attributable to intestinal nematode infections. PARASITOLOGY-CAMBRIDGE-, 1994. 109: p. 373-373.
  2. Raka, S.C., et al., ASSESSMENT OF PHYTOCHEMICAL, CYTOTOXIC, ANTHELMINTIC AND THROMBOLYTIC ACTIVITY OF MIKANIA MICRANTHA LEAVES: A NEW ADDITION IN PHYTOMEDICINE. ASSESSMENT, 2019. 1: p. 246-255.
  3. Robinson, M. and X. Zhang, The world medicines situation 2011. Traditional Medicines: global situation, issues and challenges. Geneve: World Health Organization, 2011.
  4. Rates, S.M.K., Plants as source of drugs. Toxicon, 2001. 39(5): p. 603-613.
  5. Friedman, P.A. and E.G. Platzer, Interaction of anthelmintic benzimidazoles and benzimidazole derivatives with bovine brain tubulin. Biochimica et Biophysica Acta (BBA)-General Subjects, 1978. 544(3): p. 605-614.
  6. Kohler, P. and R. Bachmann, Intestinal tubulin as possible target for the chemotherapeutic action of mebendazole in parasitic nematodes. Molecular and biochemical parasitology, 1981. 4(5-6): p. 325-336.
  7. Lacey, E., The role of the cytoskeletal protein, tubulin, in the mode of action and mechanism of drug resistance to benzimidazoles. International journal for parasitology, 1988. 18(7): p. 885-936.
  8. Kabir, M.S.H., et al., In vitro anthelmintic activity of Macaranga denticulata and in silico molecular docking analysis of its isolated compounds with tubulin.
  9. Horton, J., Albendazole: a review of anthelmintic efficacy and safety in humans. Parasitology, 2000. 121(S1): p. S113-S132.
  10. Goswami, G.N., et al., Anthelmintic Activityof Crude Extract of Zingiber Officinale (Zingiberaceae). Indian Research Journal of Pharmacy and Science, 2017. 4(4): p. 1201-1204.
  11. Goswami, G.N., et al., ANTHELMINTIC ACTIVITYOF CRUDE EXTRACT OF ZINGIBER OFFICINALE (ZINGIBERACEAE).
  12. Babu, C.R., Herbaceous Flora of Dehra Dun. 1977.
  13. Rahman, M.A. and M. Yusuf, Zingiber salarkhanii (Zingiberaceae), a new species from Bangladesh. Bangladesh Journal of Plant Taxonomy, 2013. 20(2): p. 239-242.
  14. Ganesan, S., N.R. Pandi, and N. Banumathy, Ethnomedicinal survey of Alagarkoil hills (reserved forest), Tamil nadu, India. EJournal of Indian Medicine, 2007. 1(1): p. 18-18.
  15. Padal, S., H. Ramakrishna, and R. Devender, Ethnomedicinal studies for endemic diseases by the tribes of Munchingiputtu Mandal, Visakhapatnam district, Andhra Pradesh, India. Int. J. Med. Aroma. Plant, 2012. 2: p. 453-459.
  16. Prakash, O., et al., Phytochemical composition of essential oil from seeds of Zingiber roseum Rosc. and its antispasmodic activity in rat duodenum. Journal of ethnopharmacology, 2006. 106(3): p. 344-347.
  17. Al-Amin, M., et al., Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. Journal of Research in Pharmacy, 2019. 23(3).
  18. Duwiejua, M., et al., The anti-inflammatory compounds of Polygonum bistorta: Isolation and characterisation. Planta medica, 1999. 65(04): p. 371-374.
  19. Williamson, E.M., D.T. Okpako, and F.J. Evans, Selection, Preparation and Pharmacological Evaluation of Plant Material, Volume 1. Vol. 1. 1996: John Wiley & Sons.
  20. Ajaiyeoba, E., P. Onocha, and O. Olarenwaju, In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extracts. Pharmaceutical biology, 2001. 39(3): p. 217-220.
  21. Parker, T.J. and W.A. Haswell, Textbook of zoology. 1967: Macmillan International Higher Education.
  22. Kokate, C., New Delhi: Vallabh Prakashan; 1994. Practical Pharmacognosy: p. 107-13.
  23. Raaman, N., Phytochemical techniques. 2006: New India Publishing.
  24. Patil, V. and S. Deokule, Pharmacognostic study of Chlorophytum tuberosum Baker. International journal of Ayurveda research, 2010. 1(4): p. 237.
  25. Khandelwal, K.R., Practical pharmacognosy. 2008: Pragati Books Pvt. Ltd.
  26. Berman, H.M., et al., The protein data bank, 1999–. International tables for Crystallography, 2006.
  27. Liu, Y., et al., CB-Dock: a web server for cavity detection-guided protein–ligand blind docking. Acta Pharmacologica Sinica, 2020. 41(1): p. 138-144.
  28. Goel, R.K., et al., PASS-assisted exploration of new therapeutic potential of natural products. Medicinal Chemistry Research, 2011. 20(9): p. 1509-1514.
  29. Khurana, N., et al., PASS assisted prediction and pharmacological evaluation of novel nicotinic analogs for nootropic activity in mice. European journal of pharmacology, 2011. 662(1-3): p. 22-30.
  30. Mittal, M., et al., PASS-assisted exploration of antidepressant activity of 1, 3, 4-trisubstituted-β-lactam derivatives. Bioorganic & medicinal chemistry letters, 2008. 18(20): p. 5347-5349.
  31. Amanat, M., et al., Zingiber roseum Rosc. rhizome: A rich source of hepatoprotective polyphenols. Biomedicine & Pharmacotherapy, 2021. 139: p. 111673.
  32. Raghav, D., et al., In-silico designing of novel camptothecin analogues as potent inhibitors of topoisomerase I: a molecular docking, QSAR, and ADME-T study. Letters in Drug Design & Discovery, 2016. 13(9): p. 859-868.
  33. Saleh-e-In, M.M., et al., Pharmacological effects of the phytochemicals of Anethum sowa L. root extracts. BMC complementary and alternative medicine, 2016. 16(1): p. 1-14.
  34. Jamkhande, P.G. and S.R. Barde, Evaluation of anthelmintic activity and in silico PASS assisted prediction of Cordia dichotoma (Forst.) root extract. Ancient science of life, 2014. 34(1): p. 39.
  35. Sahu, A.K., C. Panda, and B.S. Nayak, Determination of phytochemical and anthelmintic activity of rhizome of Zingiber zerumbet. J Pharm Adv Res, 2018. 1: p. 399-402.
  36. Prasanth, D., et al., Anthelmintic activity of Mansoa alliacea against Pheretima posthuma: In vitro and In silico approach. Thai Journal of Pharmaceutical Sciences, 2020. 44(3).
  37. Sreejith, M., et al., Phytochemical, Anti-oxidant and Anthelmintic activities of various leaf extracts of Flacourtia sepiaria Roxb. Asian Pacific journal of tropical biomedicine, 2013. 3(12): p. 947-953.
  38. Maisale, A., et al., Anthelmintic activity of fruit pulp of Cordia dichotoma. International Journal of Research in Ayurveda and Pharmacy (IJRAP), 2010. 1(2): p. 597-600.
  39. Bate-Smith, E., The phenolic constituents of plants and their taxonomic significance. I. Dicotyledons. Botanical Journal of the Linnean Society, 1962. 58(371): p. 95-173.

International Journal of Scientific Research in Chemistry

Downloads

Published

2022-01-31

Issue

Volume 7, Issue 1, January-February-2022

Section

Research Articles

License

Copyright (c) IJSRCH

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Muhammed Amanat, Muhammad Tawhid, A.F.M Shahid-Ud-Daula, " Anthelmintic activity of Zingiber roseum rhizomes against Pheretima posthuma: In vitro and in silico approach, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 7, Issue 1, pp.01-15, January-February-2022.