Therapeutic Potential of Silver Nano Particles Synthezised from Azadirachta Indica Against Methicillin-Resistant Staphylococcus Aureus

Authors

  • Shamsuddeen Salisu Tashena  Department of microbiology and biotechnology, Federal University Dutse, Nigeria

Keywords:

Antimicrobial Activity, Silver Nanoparticles, AgNPs, MRSA

Abstract

The increasing rate of drug resistance associated with methicillin resistant Staphylococcus aureus is not only a problem in the clinical sector but also in animal’s disease treatment and management. Methicillin resistant Staph. aureus is now a leading cause of staphylococcal infections in human and animals. Silver is known for its antimicrobial activity, silver nanoparticles are gaining great importance due to their antimicrobial activities. “Green technology” is the use of various plant materials for the biosynthesis of nanoparticles, as it does not involve any harmful chemicals. Bioactive compounds such as flavonoids, terpenoids etc present in plant extracts have made them best material for the green synthesis of nanoparticles. The present work leads to the synthesis of silver nanoparticles from 1 mM AgNO3 solution through various concentration of aqueous leaf extract of Azadirachta indica reducing as well as capping agent and the evaluation of its therapeutic potential against MRSA. The leaf extracts of Azadirachta indica were prepared using water and is screened for antimicrobial active principles. The phytochemical screening of the leaf extracts revealed the presence of alkaloids, flavonoids and saponins. The activities were tested against bacterial pathogen. The results evidently show that the inclusion of Azadirachta indica extracts improves the solubility of AgNPs, which led to a significant enhancement in the toxicity of the NPs against the assessed microorganism All the three synthesized nanoparticles (S1, S2, and S3) inhibit the bacteria at various concentration of 0.1ml, 0.2ml, and 0.3ml. The result for S1 at the said concentration were 9mm, 10mm, and 11mm. the result for S2 were 11mm, 12mm, and 13mm. The result for S3 were 12mm, 13mm and 15mm. The minimum inhibitory concentration for AgNPs against MRSA were found to be at the highest concentration of 10-1(v/v) on all the three replica of the synthesized AgNPs i.e S1, S2 and S3. The zones of inhibition are 9mm, 11mm and 13mm respectively. Nanoparticles of silver could inhibit the growth of MRSA in low densities. In well diffusion method, results revealed that the increase in the concentration of the nanoparticles of silver enlarges the diameter of the inhibited growth zone of the MRSA. The diameter of the inhibited growth zone greatly depends on the dose of nanoparticles of silver used.

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International Journal of Scientific Research in Chemistry

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Published

2022-08-05

Issue

Volume 7, Issue 4, July-August-2022

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Research Articles

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How to Cite

[1]
Shamsuddeen Salisu Tashena, " Therapeutic Potential of Silver Nano Particles Synthezised from Azadirachta Indica Against Methicillin-Resistant Staphylococcus Aureus, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 7, Issue 4, pp.14-26, July-August-2022.