Scientific Standard Validation and HPTLC. Finger printing studies of Polyherbal Unani Formulation Jawarish-e-Usquf

Authors

  • Pawan Kumar Sagar  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • A.S. Khan  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • M.W.Ahmed  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • R.P.Meena  Central Council for Research in Unani Medicine,(Under Ministry of AYUSH., Govt. of India),61-62, Institutional Area, Janakpuri, New Delhi, India
  • S.A. Ansari  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • S. Sajwan  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • S. Kashyap  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India

Keywords:

Jawarish-Usquf, Physico-chemical quality, Quality Control and Assurance parameters, TLC/ HPTLC fingerprinting and Unani Compound drug.

Abstract

Standardization and product validation is used to describe all measures under taken during the manufacturing process and quality control and quality assurance of drug leading to its reproducible quality. Therefore we need to develop standard validation techniques to standardize and validate of the herbal formulations. The drug Jawarish-Usquf is therapeutically useful in the treatment of Laqwa (Facial / Bell’s palsy), Qulanj (intestinal pain, coli), Nafkh-i-Shikam (flatulence), Bawasir (piles / hemorrhoids), Waja’ al-Khasira (low backache) Waja’ al-Zahr(bachache), Qay(vomiting) Action wise Munaqqi-e-Asab, (Nervine Cleansers). The drug Jawarish-Usquf was prepared in three different batches as per the guidelines of National Formulary of Unani Medicine(Part-IV) and UPI.(Part-II, Edition-IInd). Present research study is aimed to evaluate the product quality validation using physico-chemical parameters; HPTLC fingerprinting as per WHO guidelines of analyzed parameters. The physico-chemical average reading data’s of every III Batches of test samples showed that the drug contain LOD/ Moisture ,w/w- (15.14%,14.44%, 14.63%), Total ash, w/w- (1.34%,1.37%,1.42%), Acid in-soluble ash, w/w-(0.015%,0.014%,0.016%), Alcohol and water soluble extractive matter, w/v- (60.21%, 60.54%,61.29%) & (70.78%,71.24%, 70.58%), pH(1% solution) (5.77,5.83,5.69), pH(10% solution) (4.66,4.81, 4.74), Bulk density ,gm/ml - (1.615,1.606,1.603),Reducing Sugar (53.78, 53.36, 52.97) and Non-Reducing Sugar (6.32,6.79,6.27) the HPTLC finger prints showed various spots at 254nm, 366nm and visible light (V-S reagent).The validation of quality control studies revealed the absence of Microbial load, Aflatoxins, Heavy metal and Pesticide residues, The evaluated validated quality standards will be very useful for referential support, validation of the standards of Jawarish-Usquf, pharmaco-vigilance and providing the quality medicine to needful human being.

References

  1. Asma Sattar Khan, Shoeb Ahmed Ansari, Sonali Sajwan, Pawan Kumar Sagar, Reesha Ahmed and Syed Arshad Ali Hashmi.2022.Pharmacognostical and HPTLC Evaluation of Jawarish-e-Ood Tursh: An Anti-emetic Unani Formulation. European Journal of Biomedical and Pharmaceutical Sciences ; 7(12): 210-218. (ISSN: 2349-8870); DOI: https://www.ejbps.com/ejbps/abstract_id/8544
  2. Anonymous.2022. National Formulary of Unani Medicine Part IV, Edition -IInd, published by PCIM&H, Ghaziabad UP, Ministry of Ayurveda, Yoga & Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Government of India, New Delhi. Part-IV,(IInd Edition):92.; DOI: https://pcimh.gov.in/WriteReadData/RTF1984/1536753955.pdf
  3. Pawan Kumar Sagar, R Murugeswaran, R.P. Meena, M.W. Ahmed, S.A. Ansari, S. Khair and S A A Hashmi.2020.Standardization and HPTLC Finger Printing Studies of Poly Herbal Formulation - Itrifal Haamaan. International Journal of Ayurveda and Pharmaceutical Chemistry;12(3):220-232.(e-ISSN: 2350-0204); DOI: https://oaji.net/articles/2021/1791-1632827247.pdf
  4. Pawan Kumar Sagar, R Murugeswaran, R.P. Meena, A.S. Khan, S. Mageswari, P.M.D. Sri, S. Sajwan, S.G. Ahmad.2020.Standardization and Monographs development, HPTLC .Finger printing research studies of Poly Herbal Formulation-Habb-e-Nishat Jadeed. European Journal of Biomedical and Pharmaceutical Sciences ; 7(12): 210-218. (ISSN: 2349-8870);DOI:https://storage.googleapis.com/journal-uploads/ejbps/article_issue/volume_7_december _issue_12/1606466607.pdf
  5. Sagar, P.K., Murugeswaran, R., Meena, R., Mageswari, S., and Sri, P.Meera Devi, Khair, S.2020. Standardization and HPTLC. Fingerprinting study of Poly Herbal Unani Formulation-Habb-e-Sara Khas. International Journal of Traditional and Complementary Medicine,5(21),1-13.;DOI: https://escipub.com/ijtcm-2020-01-2805/
  6. Meena, R.P., Verma, S.C., Mageswari, S., Sri Devi, P.M., Khan, A.S., Ansari, S.A., Arfin ,S. ,Aminuddin.2016. Scientific validation of Unani Formulation : Majoon-e-Hamal Ambari Alwi Khani. Hippocratic Journal of Unani Medicine,11(1):47-60. DOI: https://www.ccrum.res.in/writereaddata/UploadFile/Hippocratic13_1792.pdf
  7. Bahuguna Y, Zaidi S, Neeraj Kumar and Kailash Rawat.,2014. Standardization of Polyherbal Marketed Formulation Triphala Churna, Research and Review. Journal of Pharmacognosy and Phytochemistry 2 (3):28-35.
  8. Kunle, O.F., Egharevba, H.O., Ahmadu, P.O., 2012. Standardization of Herbal Medicines :The Review . Int. J Biodivers. Conserve 4:101-112.
  9. Yadav P, Mahour Y and Ashok Kumar.,2011. Standardization and Evaluation of Herbal Formulations, Journal of Advanced Laboratory Research in Biology2(4):161-166.
  10. Anonymous.,2011. National Formulary of Unani Medicine, Part-VI, Govt. of India, Ministry of Health & Family Welfare, Dept. of AYUSH. New Delhi,(2.19):75.
  11. Anonymous.,2009. The Unani Pharmacopeia of India, Part-I, Vol-VI, published by Govt. of India, Ministry of Health & Family Welfare, Dept. of AYUSH. New Delhi: pp.31-32 ,40-41,68-69,72-73 and 88-89.
  12. Anonymous, 2008. The Ayurvedic Pharmacopeia of India, Part-I, Vol-VI, published by Govt. of India, Ministry of Health & Family Welfare, Dept. of AYUSH. New Delhi
  13. Anonymous.,2008. The Unani Pharmacopeia of India, Part-I, Vol-V, published by Govt. of India, Ministry of Health & Family Welfare, Dept. of AYUSH. New Delhi: pp.80-81,105.
  14. Anonymous.,2007. The Unani Pharmacopoeia of India, Part-I, Vol.-I, Govt. of India, Min. of Health & Family Welfare, New Delhi: pp.5-6,26,78 and 88.
  15. Anonymous.,2007.The Unani Pharmacopoeia of India, Part-I, Vol.-II, Govt. of India, Min. of Health & Family Welfare, New Delhi: pp.29,45-46, 51-52,81-82, 89-90 and 91-92.
  16. Anonymous.,2007. The Unani Pharmacopeia of India, Part-I, Vol.-IV, Govt. of India, Min. of Health & Family Welfare, New Delhi: pp.38,49-50.
  17. Kabeeruddin, Hakim M. (2006). AlQarabadeen (Urdu), Central Council for Research in Unani Medicine, Janakpuri, New Delhi.
  18. Anonymous.,2006, Official Methods of Analysis(AOAC), International, Horwitz W, Latimer G W,18thEdn. AOAC International; chapter 26,p.17.
  19. Anonymous.,2006. National Formulary of Unani Medicine, Part-IV,(First Edition),2.19, Govt. of India, Ministry of Health & Family Welfare, Dept. of AYUSH. New Delhi:75.
  20. Patel, P.M., Patel, N.M., Goyal, R.K.,2006. Quality control of herbal products, The Indian Phamacist,5(45):26-30.
  21. Anonymous, 2005. Global Atlas of Traditional, Complementary and Alternative Medicine, Vol.1 and Vol. 2. WHO, Geneva. EMEA, 2005. Guidelines on Quality of Herbal Medicinal Products/Traditional Medicinal Products, EMEA/CVMP/814OO Review. London: European Agency for the Evaluation of Medicinal Products (EMEA).
  22. Anonymous, 2005. Official Methods of Analysis of AOAC International, Horwitz W, Latimer GW(edn). 18th Edn. AOAC International: Maryland, chapter 3, p. 10-11, chapter 10 p.18-23 and chapter-26, p.17.
  23. Anonymous, 2002. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, WHO, Geneva.
  24. Anonymous, 2000. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, WHO, Geneva.
  25. Anonymous.,1998. Quality Control Methods for Medicinal Plant Materials. World Health Organization, Geneva: pp 25-28.
  26. Anonymous.,1997. Official Analytical Methods of the American Spice Trade Association (ASTA), Inc. 4thedn. New Jersey: pp 149-152.
  27. Sethi, P.D., 1996. High Performance Thin Layer Chromatography, 1st Edn., Vol. X. CBS Publishers and Distributers, New Delhi, pp.1-56.
  28. Wagner, H and Biadi, S.,1996. Plant Drug Analysis - A Thin Layer Chromatography Atlas, Springer-Verlag, 2ndEdn., Germany.
  29. Siddqui Hakim MA.,1995. Format for the pharmacopoeial analytical standards of compound formulation, workshop on standardization of Unani drugs, 24-25th January (appendix), Central Council for Research in Unnai Medicine, New Delhi.
  30. Anonymous.,1991. Physico-chemical standards of Unani Formulations, Part-II, CCRUM, Min. of Health and Family Welfare, New Delhi: pp.15-57
  31. Anonymous., 1990. The Ayurvedic Pharmacopoeia of India, Part –I,Vol.-1,Govt. of India, Min. of Health & Family Welfare, New Delhi: pp.120-121 and 142-143.
  32. Anonymous, 1987. Physico-chemical standards of Unani Formulations, Part - II.CCRUM , Ministry of Health & Family Welfare, New Delhi, pp. 9-54;300-317.
  33. Anonymous.,1987. Physico-chemical standards of Unani Formulations, Part-II, CCRUM, Min. of Health and Family Welfare, New Delhi: pp.9-54
  34. Wallis, R.E., 1987. Text Book of Pharmacognosy, 5th Edition. CBS Publisher & Distributors , Delhi, pp. 494-496.
  35. Anonymous.,1986. Physico-chemical standards of Unani Formulations, Part-II, CCRUM, Min. of Health and Family Welfare, New Delhi: pp.27-42
  36. Wagner, H. and Bladt, S., 1984. Plant Drug Analysis - A Thin Layer Chromatography Atlas. Springer-Verlag, 2nd Edn., Germany.
  37. Saxena, S.C. and Yadav, R.S., 1983. A new plant extract to suppress the population of yellow fever and dengue vector Aedesaegyptii L. Current Science 52: 713-715.
  38. Anonymous, 1981. National Formulary of Unani Medicine, Part-I. Central Council for Research in Unani Medicine, Ministry of Health and Family Welfare, Govt. of India, p. 134.
  39. Johansen, D.A., 1940. Plant Microtechnique. Mc. Graw Hill Book Company Inc., New York and London, pp. 181-186.

International Journal of Scientific Research in Chemistry

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Published

2023-07-30

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Volume 8, Issue 4, July-August-2023

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Research Articles

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[1]
Pawan Kumar Sagar, A.S. Khan, M.W.Ahmed, R.P.Meena, S.A. Ansari, S. Sajwan, S. Kashyap, " Scientific Standard Validation and HPTLC. Finger printing studies of Polyherbal Unani Formulation Jawarish-e-Usquf, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 8, Issue 4, pp.01-13, July-August-2023.