Scientific Standard Validation and HPTLC. Finger printing studies of Polyherbal Unani Formulation Majoon-e-Maddat-ul-Hayat Jadwari

Authors

  • Pawan Kumar Sagar  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • A.S. Khan  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • R. P. Meena  Central Council for Research in Unani Medicine,(Under Ministry of AYUSH., Govt. of India),61-62, Institutional Area, Janakpuri, New Delhi, India
  • S. A. Ansari  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • S. Sajwan  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • S. Kashyap  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India
  • R. Ahmed  Drug Standardization Research Institute, (Under CCRUM, Ministry of AYUSH., Govt. of India), PCIM&H Campus, IInd Floor, Kamla Nehru Nagar, Ghaziabad, U.P., India

Keywords:

Majoon-e-Maddat-ul-Hayat Jadwari, Physico-chemical quality, Quality Control and Assurance parameters, TLC/ HPTLC fingerprinting and Unani Compound drug.

Abstract

The polyherbal drug Majoon-e-Maddat-ul-Hayat Jadwari has been used as a traditional and alternative medicine to treat and cure therapeutically in the treatment of Nisyan (Amnesia), Salasul Baul (Urinary Incontinence), Waja-ul-Kulya (Renal pain /Nephralgia), Waj-ul-Qutn (Lumbago), Waj-ul-Mafasil (Rheumatoid / Poly Arthritis) and has also been used Action wise Muqawwi-e-Asab, (Nervine Tonic), Muwallid-i-Mani (Spermatogenic), Hazim (Digestive), Daf-e-waja (Analgesic), Muqawwi-i-Bah (Aphrodisiac). In the present research study is aimed to evaluate the product drug standardization and quality validation using physico-chemical parameters; HPTLC fingerprinting, essential WHO and AYUSH Pharmacopeial parameters as per WHO and AYUSH guidelines protocols of analyzed parameters. Drug Standardization and product validation is very essential tools for validation and measured the batch to batch variation or uniformity of ASU herbal product. Therefore we need to develop standard validation techniques to standardize and validate of the poly herbal formulation. The drug MEMUHJ was prepared in three different batches as per the guidelines of Classical text / literatures of NFUM Vol,IVth Edition Ist and IInd. The physico-chemical average reading data’s of every III Batches of test samples showed that the drug contain LOD/ Moisture ,w/w- (6.42%,6.69%,6.56%), Total ash, w/w- (1.66%,1.78%,1.66%), Acid in-soluble ash, w/w-(0.266%,0.323%,0.318%), Alcohol (ASEM) and Water (WSEM) soluble extractive matter, w/v- (53.66%,54.66%,55.04%) & (73.48%,74.01%, 74.41%) and Alcohol soluble successive extractive value (ASSEV). w/v- 54.92%,54.96%,54.90% , Chloroform soluble successive extractive value (CSSEV). w/v- 16.85%,16.72%,16.82%, pH (1% solution) (4.58,4.55, 4.51),pH(10% solution) (5.45,5.57, 5.75), Bulk density ,gm/ml - (1.525,1.515,1.518),Reducing Sugar (54.31, 55.40, 54.80) and Non-Reducing Sugar (23.43,23.52,23.50) the HPTLC finger prints showed various spots at 254nm, 366nm and visible light (V-S reagent). The validation of quality control study revealed the absence of Microbial load, Aflatoxins, Heavy metal and Pesticide residues, The evaluated validated quality standards will be very useful for referential support, pharmaco-vigilance and validation of the standards of MEMUHJ providing the quality medicine to needful human being.

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International Journal of Scientific Research in Chemistry

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Published

2023-07-30

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Volume 8, Issue 4, July-August-2023

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Research Articles

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[1]
Pawan Kumar Sagar, A.S. Khan, R. P. Meena, S. A. Ansari, S. Sajwan, S. Kashyap, R. Ahmed, " Scientific Standard Validation and HPTLC. Finger printing studies of Polyherbal Unani Formulation Majoon-e-Maddat-ul-Hayat Jadwari, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 8, Issue 4, pp.14-29, July-August-2023.