Mass Transfer Effects and Performance of Immobilized Enzymes
Keywords:
Immobilized enzymes, Cross-linked crystals, Mass transfer, Performance, Modified enzyme, Enzyme powder.Abstract
Immobilized enzymes are currently the object of considerable interest. This is due to the expected benefits over soluble enzymes or alternative technologies. The number of applications of immobilized enzymes is increasing steadily, however, experimental investigations have produced unexpected results such as a significant reduction or even an increase in activity compared with soluble enzymes. Thus, cross-linked crystals of subtilisin showed 27 times less activity in the aqueous hydrolysis of an amino acid ester compared to equal amounts of soluble enzyme. On the other hand, in the application of lipoprotein lipase in the solvent-mediated synthesis of esters there was a 40-fold increase in activity using immobilized or otherwise modified enzyme preparations as compared to enzyme powder.
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