Mass Transfer Effects and Performance of Immobilized Enzymes

Authors

  • Akshay R.Yadav  Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Dist- Sangli, Maharashtra, India
  • Dr. Shrinivas K. Mohite  Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Dist- Sangli, Maharashtra, India

Keywords:

Immobilized enzymes, Cross-linked crystals, Mass transfer, Performance, Modified enzyme, Enzyme powder.

Abstract

Immobilized enzymes are currently the object of considerable interest. This is due to the expected benefits over soluble enzymes or alternative technologies. The number of applications of immobilized enzymes is increasing steadily, however, experimental investigations have produced unexpected results such as a significant reduction or even an increase in activity compared with soluble enzymes. Thus, cross-linked crystals of subtilisin showed 27 times less activity in the aqueous hydrolysis of an amino acid ester compared to equal amounts of soluble enzyme. On the other hand, in the application of lipoprotein lipase in the solvent-mediated synthesis of esters there was a 40-fold increase in activity using immobilized or otherwise modified enzyme preparations as compared to enzyme powder.

References

  1. Gloger M, Tischer W (1983) In: Bergmeyer HU, Bergmeyer J, Graßl M (eds) Methods of enzymatic analysis, 3rd edn.VCH, Weinheim, vol 1, pp 142–163
  2. Tischer W (1995) In: Drauz K,Waldmann H (eds) Enzyme catalysis in organic synthesis. VCH, Weinheim, pp 73–87.
  3. Lee YY, Tsao GT (1974) J Food Sci 39:667.
  4. Yamane T (1981) J Ferment Technol 59:37.
  5. Kasche V (1983) Enzyme Microb Technol 5:2.
  6. Schlothauer RC (1996) Thesis TU Hamburg-Harburg Engasser J-M, Horvath C (1974) Biochemistry 13:3845.
  7. Horvath C, Engasser J-M (1974) Biotechnol Bioeng 16:909.
  8. Carleysmith SW, Dunnill P, Lilly MD (1980) Biotechnol Bioeng 22:735
  9. Horvath C, Engasser J-M (1973) Ind Eng Chem Fundam 12:229.
  10. McLAren AD, Packer L (1970) Adv Enzymol 33:245.
  11. Goldstein L, Levin Y, Katchalski E (1964) Biochemistry 3:1913.
  12. Trevan MD (1980) Immobilized enzymes – an introduction and applications in biotechnology. Wiley, New York, pp 11–55
  13. Halwachs W, Wandrey C, Schügerl K (1978) Biotechnol Bioeng 20:541.
  14. Ruckenstein E, Sasidhar V (1984) Chem Eng Sci 39:1185
  15. Kasche V, Bergwall M (1977) In: Salmona M, Saranio M, Garattini S (eds) Insolubilized enzymes. Raven Press,New York, pp 77–86.
  16. Rolinson GN (1988) J Antimicrob Chemother 22:5.
  17. Liou JK, Rousseau I (1986) Biotechnol Bioeng 28:1582.
  18. Bailey JE, Chow MTC (1974) Biotechnol Bioeng 16:1345.
  19. Ruckenstein E, Rajora P (1985) Biotechnol Bioeng 27:807.
  20. Dickinson M, Fletcher PDI (1988) Enzyme Microb Technol 11:55
  21. Klibanov AM (1997) Trends Biotechnol 15:97
  22. Carrea G, Ottolina G, Riva S (1995) Tibtech 13:63.
  23. Tsai S-W,Dordick JS (1996) Biotechnol Bioeng 52:296.
  24. Zacharis E, Moore B, Halling PJ (1997) J Am Chem Soc 119:12396.
  25. Tischer W, Giesecke U, Lang G, Röder A, Wedekind F (1992) Ann N Y Acad Sci 672:502
  26. Schmid RD, Verger R (1998) Angew Chem 110:1694.
  27. Naik SS, Karanth NG (1978) J Appl Chem Biotechnol 28:569.
  28. Lalonde JJ, Govardhan CP, Khalaf N, Martinez AG, Visuri KL, Margolin A (1995) J Am Chem Soc 117:6845.
  29. Persichetti RA, Clair NLS, Griffith JP, Navia AL, Margolin AL (1995) J Am Chem Soc 117:2732.
  30. Nagayasu T, Miyanaga M, Tanaka T, Sakiyama T, Nakanishi K (1997) Biotechnol Bioeng 43:1118

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Published

2020-10-30

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Section

Research Articles

How to Cite

[1]
Akshay R.Yadav, Dr. Shrinivas K. Mohite, " Mass Transfer Effects and Performance of Immobilized Enzymes, International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 5, Issue 5, pp.51-56, September-October-2020.