In vitro Studies on the Inhibition of ?-amylase and ?-glucosidase by Methanolic Extract of Barleria Prionitis L. and Psidium guajava

Authors

  • Akshay R. Yadav  Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Dist- Sangli, Maharashtra, India
  • Dr. Shrinivas K. Mohite   Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Dist- Sangli, Maharashtra, India

Keywords:

Barleria Prionitis, Psidium guajava, Alpha-amylase, Alpha-glucosidase, Antidiabetic.

Abstract

The management of the blood glucose level is a critical strategy in the control of diabetes complications. There are many and diverse therapeutic strategies in the management of Type II diabetes. The inhibition of carbohydrate hydrolyzing enzymes such as ?-amylase can be an important strategy to lower postprandial blood glucose levels. Such inhibitors which find application in the clinical practice for management of diabetes are known to be associated with various gastrointestinal side effects. Therefore, it is the need of time to identify and explore the amylase inhibitors from natural sources having fewer side effects. In the present study, 50% volume per volume methanolic extracts of Barleria Prionitis and psidium guajava subjected to in vitro analysis of antidiabetic effect by alpha amylase and alpha-glucosidase inhibitory assay. Inhibitory activity of the methanolic extract of the plant individually against alpha-amylase enzyme and alpha-glucosidase enzyme were examined in different concentrations (3.90–500 ?g/mL), where acarbose used as a positive control. The percentage inhibition of Barleria Prionitis showed the highest alpha-amylase and alpha-glucosidase inhibitory activity. Half maximal inhibitory concentration value Barleria Prionitis was found for alpha amylase and alpha glucosidase inhibition. This study suggests that the methanolic extract of all two plants have antidiabetic property, among these three plants Barleria Prionitis showed potent enzyme inhibition as compared to other plant extracts and standard acarbose.

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2020-02-27

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Research Articles

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[1]
Akshay R. Yadav, Dr. Shrinivas K. Mohite , " In vitro Studies on the Inhibition of ?-amylase and ?-glucosidase by Methanolic Extract of Barleria Prionitis L. and Psidium guajava , International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 5, Issue 1, pp.50-58, January-February-2020.