Plant Based Green Synthesis, Characterization and Antimicrobial Investigation of Silver Nanoparticles from Khaya senegalensis Aqueous Leaf Extract

Authors

  • Mela Yoro  Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, P.M.B 0182 Gombe State, Nigeria
  • Bertha Abdu Danja  Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, P.M.B 0182 Gombe State, Nigeria
  • Rebeccah Bethuel  Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, P.M.B 0182 Gombe State, Nigeria
  • Japhet Joshua  Department of Science Laboratory Technology, Federal Polytechnic, Kaltungo, Gombe, Nigeria
  • Joyous W.K. Jonah  Department of Science Laboratory Technology, Federal Polytechnic, Kaltungo, Gombe, Nigeria
  • T.A. Suleiman  Abubakar Tafawa Balewa University College of Medical Sciences, Bauchi State

Keywords:

Antimicrobial, Characterization, Green synthesis, Khaya senegalensis, Silver Nanoparticles

Abstract

In this research article, Silver nanoparticles were synthesized from Khaya senegalensis leaf extracts via green route. Spectroscopic study revealed color change of the solution from yellow to light brown within 25 min of addition of the AgNO3 against leaf aqueous extract with constant stirring. Beyond this time, no further change in color after the nucleation of the metal ions indicating that phytoconstituents of Khaya senegalensis resulted in the reduction of Ag+ to Ag0, a phenomenon that could be attributed to the surface Plasmon excitement of AgNPs. The bio fabricated silver nanoparticles were characterized using UV – Visible, FTIR and RXD so as to be certain of its formation before being deployed in the antimicrobial studies. The UV-Vis spectral analysis of the AgNPs from the leaf extract showed maximum absorbance of 2.01AU at a corresponding wavelength (? max) of 500nm. The X-Ray Diffraction patterns of green synthesized AgNPs showed that the structure of the nanoparticles under research have face centered cubic (fcc) and spinel like structures with biosynthesized AgNPs having the average particle size of 65.5nm. The antimicrobial studies of Silver nanoparticles were conducted against B. subtilis, K. pneumonia (gram +ve bacteria) and two fungi, A. niger and C. albicans. Different concentrations of 100, 200, 300, 400 and 500?g/L of Silver nanoparticles were tested against each pathogen. The inhibition zone increases generally with increase in concentrations of silver nanoparticles. At higher concentration of 500?g/L, the zones of inhibition were in the following order; 18.5mm, 26.3mm, 23.5mm, and 24.4mm for Bacillus subtilis, Klebsiella pneumonia, Aspergillus niger and Candida albicans respectively. For each concentration investigated, C. albicans, demonstrated higher zone of inhibition as opposed to all other pathogens under investigation. The results of this research therefore indicated that Silver Nanoparticles synthesized from Khaya senegalensis plant extracts demonstrated potent antimicrobial activity on the selected pathogenic microbes, hence be used as antimicrobial agent against the organisms in question.

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International Journal of Scientific Research in Chemistry

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Published

2023-09-30

Issue

Volume 8, Issue 5, September-October-2023

Section

Research Articles

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How to Cite

[1]
Mela Yoro, Bertha Abdu Danja, Rebeccah Bethuel, Japhet Joshua, Joyous W.K. Jonah, T.A. Suleiman, " Plant Based Green Synthesis, Characterization and Antimicrobial Investigation of Silver Nanoparticles from Khaya senegalensis Aqueous Leaf Extract , International Journal of Scientific Research in Chemistry(IJSRCH), ISSN : 2456-8457, Volume 8, Issue 5, pp.01-10, September-October-2023.